Prolonged and continuous use of topical steroid can cause "red face in dermatology" which presents clinically as diffused erythema with/without papules, pustules and sometimes nodules with telangiectasia. Treatment is difficult as there is rebound phenomenon with discontinuation of the topical steroid. Gradual tapering with complete cessation of the topical steroid and addition of oral anti-inflammatory antibiotics and/or topical antibiotics are usually recommended to get a good clinical result. The limitation of this study is the absence of histopathologic studies, which even though may not be diagnostic and specific, could have been done to rule out other pathologies. Also, contact dermatitis to topical steroids was not ruled out and so some patients with contact dermatitis to topical steroids may have been included in the study. This needs to be ruled out by doing patch test with topical corticosteroid series.
10 Volumina of the supernatant comprising rhamnolipids was passed over an amberlit XAD-8 of XAD-2 column (Rohm & Haas). The column was washed with water. The rhamnolipids were eluted using 100% MeOH. The fractions comprising rhamnolipids were evaporated and subsequently added to pure N HCl was added to precipitate the rhamnolipids. Precipitated rhamnolipids were centrifuged at 3000 rpm for 10 minutes. The rhamnolipids now precipitated were washed using pure water and subsequently centrifuged at 3000 rpm for 10 minutes. Using 1/10N NaOH the pH of the precipitated lipids were adjusted to . After lyophilization 10 g of the lyophilized preparation was dissolved in 50 ml propanol and applied to a silica column (Waters HPLC, volume 500 ml) equilibrated with hexane. Using 5 l propanol impurities were eluted. The rhamnolipids were eluted using a solvent mixture comprising propanol-25% NH 4 OH (4:1). The active fractions were evaporated and dissolved in water, again precipitated in 1N HCl, centrifuged at 3000 rpm, and the precipitants were adjusted to pH using NaOH, and lyophilized.